Insights of CRISPR-Cas systems in stem cells: progress in regenerative medicine.

Regenerative medicine, a therapeutic approach using stem cells, aims to rejuvenate and restore the normalized function of the cells, tissues, and organs that are injured, malfunctioning, and afflicted. This influential technology reaches its zenith when it is integrated with the CRISPR-Cas (clustered regularly interspaced short palindromic repeats-CRISPR associated) technology of genome editing. This tool acts as a programmable restriction enzyme system, which targets DNA as well as RNA and gets redeployed for the customization of DNA/RNA sequences. The dynamic behaviour of nuclear manipulation and transcriptional regulation by CRISPR-Cas technology renders it with numerous employment in the field of biologics and research. Here, the possible impact of the commonly practiced CRISPR-Cas systems in regenerative medicines is being reviewed. Primarily, the discussion of the working mechanism of this system and the fate of stem cells will be scrutinized. A detailed description of the CRISPR based regenerative therapeutic approaches for a horde of diseases like genetic disorders, neural diseases, and blood-related diseases is elucidated.

Efficiency of microbial fuel cells in the treatment and energy recovery from food wastes: Trends and applications - A review.

The rising global population and their food habits result in food wastage and cause an obstacle in its treatment and disposal. Due to the rapid shift in the lifestyle of the human population and urbanization, almost one-third of the food produced is wasted from various sectors like domestic sources, agricultural sectors, and industrial sectors. These food resources squandered are rich in organic biomolecules which can cause complications upon direct disposal in the environment. Conventional disposal methods like composting, landfills and incineration demand high costs besides causing severe environmental and health issues. To overcome these demerits of the conventional methods and to avoid the loss of rich organic food resources, there is an immediate need for a sustainable and eco-friendly solution for the valorization of the food wastes. Microbial fuel cells (MFCs) are gaining attention, due to their ideal approach in the production of electricity and parallel treatment of organic food wastes. The MFCs are significant as an innovative approach using microorganisms and oxidizing the organic food wastes into bio-electricity. In this review, the recent advancements and practices of the MFCs in the field of food waste treatment and management along with electricity production are discussed. The major outcome of this work highlights the setting up of MFC for the treatment of higher volumes of food waste residues and enhancing the bioelectricity production in an optimal condition. For further improvements in the food waste treatments using MFCs, greater understanding and more research needs are to be focused on the commercialization, different operational modes, operational types, and low-cost fabrication coupled with careful examination of scale-up factors.

Chemico-nanotreatment methods for the removal of persistent organic pollutants and xenobiotics in water - A review.

While the technologies available today can generate high-quality water from wastewater, the majority of the wastewater treatment plants are not intended to eliminate emerging xenobiotic pollutants, pharmaceutical and personal care items. Most endocrine disrupting compounds (EDCs) and personal care products (PPCPs) are more arctic than most regulated pollutants, and several of them have acid or critical functional groups. Together with the trace occurrence, EDCs and PPCPs create specific challenges for removal and subsequent improvements of wastewater treatment plants. Various technologies have been investigated extensively because they are highly persistent which leads to bioaccumulation. Researchers are increasingly addressing the human health hazards of xenobiotics and their removal. The emphasis of this review was on the promising methods available, especially nanotechnology, for the treatment of xenobiotic compounds that are accidentally released into the setting. In terms of xenobiotic elimination, nanotechnology provides better treatment than chemical treatments and their degradation mechanisms are addressed.